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goat anti gal 4  (R&D Systems)


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    R&D Systems goat anti gal 4
    Goat Anti Gal 4, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+gal+4/Human%2FMouse%2FRat+Galectin-4+Antibody/pm38377735-68-34-38
    Average 94 stars, based on 6 article reviews
    goat anti gal 4 - by Bioz Stars, 2026-09
    94/100 stars

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    Incubation:

    Article Title: Interplay in galectin expression predicts patient outcomes in a spatially restricted manner in PDAC.
    Article Snippet: FFPE pancreas tissue Section 5 mm in depth were subjected to deparaffinisation using histoclear (Sigma) followed by incubation in decreasing concentrations of ethanol prior to antigen retrieval using a 10 mM citrate buffer at pH6 for 15 min. .. Slides were blocked using 10% normal horse serum in 2% BSA/PBS for 10 min, prior to incubation at 4◦C overnight with goat anti-Gal-1 (1:75, AF1152, R&D systems), goat anti-Gal-3 (1:75, AF1154, R&D systems) or goat anti-Gal-4 (1:20, AF1227, R&D systems) antibodies diluted in PBA with 1% BSA. .. Following 3 ×5-min washes in PBS, slides were incubated for 45 min in the dark at room temperature with donkey-anti-goat IgG AF647 (1:400, A-21447, Invitrogen).



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    Multifunctional roles of galectins in mucosal intestinal inflammation. A coordinated network of galectin family members, which exerts either anti-inflammatory or proinflammatory responses, conditions epithelial barrier maintenance and immune gut homeostasis. Galectin-1 (Gal-1) promotes the secretion of growth factors and anti-inflammatory cytokines by epithelial cells (ECs), induces apoptosis of activated Th1 and Th17 cells, inhibits secretion of proinflammatory cytokines by dendritic cells (DCs) and T lymphocytes, and favors an anti-inflammatory (M2) macrophage phenotype. Similar to Gal-1, Gal-2 displays several anti-inflammatory properties, but also promotes wound healing and tissue regeneration in ECs. In animal models of colitis, Gal-3 shows mostly proinflammatory functions, inhibiting the polarization of macrophages toward an M2 phenotype, whereas Gal-4 exhibits both anti- and proinflammatory properties within the intestinal inflamed mucosa, depending on the experimental setting analyzed. By blocking production of proinflammatory cytokines, Gal-4 prevented inflammation and favored epithelial regeneration. However, through binding to memory T-cells, Gal-4 led to T-cell activation and perpetuated intestinal inflammation.

    Journal: Frontiers in Immunology

    Article Title: Galectins in Intestinal Inflammation: Galectin-1 Expression Delineates Response to Treatment in Celiac Disease Patients

    doi: 10.3389/fimmu.2018.00379

    Figure Lengend Snippet: Multifunctional roles of galectins in mucosal intestinal inflammation. A coordinated network of galectin family members, which exerts either anti-inflammatory or proinflammatory responses, conditions epithelial barrier maintenance and immune gut homeostasis. Galectin-1 (Gal-1) promotes the secretion of growth factors and anti-inflammatory cytokines by epithelial cells (ECs), induces apoptosis of activated Th1 and Th17 cells, inhibits secretion of proinflammatory cytokines by dendritic cells (DCs) and T lymphocytes, and favors an anti-inflammatory (M2) macrophage phenotype. Similar to Gal-1, Gal-2 displays several anti-inflammatory properties, but also promotes wound healing and tissue regeneration in ECs. In animal models of colitis, Gal-3 shows mostly proinflammatory functions, inhibiting the polarization of macrophages toward an M2 phenotype, whereas Gal-4 exhibits both anti- and proinflammatory properties within the intestinal inflamed mucosa, depending on the experimental setting analyzed. By blocking production of proinflammatory cytokines, Gal-4 prevented inflammation and favored epithelial regeneration. However, through binding to memory T-cells, Gal-4 led to T-cell activation and perpetuated intestinal inflammation.

    Article Snippet: Gal-4 , Goat anti-Gal-4 antibody (1:75) (Santa Cruz Biotech, Dallas, TX, USA) , anti-goat biotinylated antibody (1:180) (Amersham Pharmacia, Buckinghamshire, UK).

    Techniques: Blocking Assay, Binding Assay, Activation Assay

    Expression of galectin-1 (Gal-1), Gal-4, and Foxp3 in response to gluten-free diet (GFD) in duodenal biopsies from celiac disease (CeD) patients. Representative micrographs of control subjects, untreated CeD patients and CeD patients subjected to gluten withdrawal (CeD-GFD patients) are shown. (A–C) Hematoxylin/eosin (H/E) staining of paraffin-embedded sections of duodenal biopsies from (A) control subjects, (B) CeD patients, and (C) CeD-GFD patients. Arrows indicate the superficial epithelium, and arrowheads indicate the glandular epithelium while asterisks denote the stroma. Bar = 20 µm. (D–F) Immunohistochemical analysis of Gal-1 expression in duodenal biopsies from control subjects (D) , CeD patients (E) , and CeD-GFD patients (F) . Bar = 20 µm. (G–I) Immunohistochemical analysis of Gal-4 expression in duodenal biopsies from control subjects (G) , CeD patients (H) , and CeD-GFD patients (I) . Bar = 20 µm. (J–L) Immunohistochemical analysis of Foxp3 + cells in biopsies from control subjects (J) , CeD patients (K) , and CeD-GFD patients (L) . Bar = 20 µm. (M) Quantification of Gal-1 expression determined by immunohistochemistry. Bars represent immunostained area corresponding to superficial (Sup) and glandular (Gl) epithelium, and stroma, in paraffin sections from duodenal biopsies from controls, untreated CeD patients and CeD-GFD patients. Evaluation of staining intensity was performed with the Image J software (NIH, Bethesda, MD, USA). One-way ANOVA Tukey test was used for multiple comparisons. ** p < 0.01, *** p < 0.001.

    Journal: Frontiers in Immunology

    Article Title: Galectins in Intestinal Inflammation: Galectin-1 Expression Delineates Response to Treatment in Celiac Disease Patients

    doi: 10.3389/fimmu.2018.00379

    Figure Lengend Snippet: Expression of galectin-1 (Gal-1), Gal-4, and Foxp3 in response to gluten-free diet (GFD) in duodenal biopsies from celiac disease (CeD) patients. Representative micrographs of control subjects, untreated CeD patients and CeD patients subjected to gluten withdrawal (CeD-GFD patients) are shown. (A–C) Hematoxylin/eosin (H/E) staining of paraffin-embedded sections of duodenal biopsies from (A) control subjects, (B) CeD patients, and (C) CeD-GFD patients. Arrows indicate the superficial epithelium, and arrowheads indicate the glandular epithelium while asterisks denote the stroma. Bar = 20 µm. (D–F) Immunohistochemical analysis of Gal-1 expression in duodenal biopsies from control subjects (D) , CeD patients (E) , and CeD-GFD patients (F) . Bar = 20 µm. (G–I) Immunohistochemical analysis of Gal-4 expression in duodenal biopsies from control subjects (G) , CeD patients (H) , and CeD-GFD patients (I) . Bar = 20 µm. (J–L) Immunohistochemical analysis of Foxp3 + cells in biopsies from control subjects (J) , CeD patients (K) , and CeD-GFD patients (L) . Bar = 20 µm. (M) Quantification of Gal-1 expression determined by immunohistochemistry. Bars represent immunostained area corresponding to superficial (Sup) and glandular (Gl) epithelium, and stroma, in paraffin sections from duodenal biopsies from controls, untreated CeD patients and CeD-GFD patients. Evaluation of staining intensity was performed with the Image J software (NIH, Bethesda, MD, USA). One-way ANOVA Tukey test was used for multiple comparisons. ** p < 0.01, *** p < 0.001.

    Article Snippet: Gal-4 , Goat anti-Gal-4 antibody (1:75) (Santa Cruz Biotech, Dallas, TX, USA) , anti-goat biotinylated antibody (1:180) (Amersham Pharmacia, Buckinghamshire, UK).

    Techniques: Expressing, Staining, Immunohistochemical staining, Immunohistochemistry, Software

    Analysis of duodenal biopsies from control subjects, untreated CeD patients, and CeD patients subjected to gluten withdrawal.

    Journal: Frontiers in Immunology

    Article Title: Galectins in Intestinal Inflammation: Galectin-1 Expression Delineates Response to Treatment in Celiac Disease Patients

    doi: 10.3389/fimmu.2018.00379

    Figure Lengend Snippet: Analysis of duodenal biopsies from control subjects, untreated CeD patients, and CeD patients subjected to gluten withdrawal.

    Article Snippet: Gal-4 , Goat anti-Gal-4 antibody (1:75) (Santa Cruz Biotech, Dallas, TX, USA) , anti-goat biotinylated antibody (1:180) (Amersham Pharmacia, Buckinghamshire, UK).

    Techniques: